1

10

20

30

40

50

60

70

80

90

100

110

120

130

Bacillus

U

U

U

G

G

U

G

A

U

G

A

U

G

G

C

A

G

A

G

A

G

G

U

C

A

C

A

C

C

C

G

U

U

C

C

C

A

U

A

C

C

G

A

A

C

A

C

G

G

A

A

G

U

U

A

A

G

C

U

C

U

C

U

A

G

C

G

C

C

G

A

U

G

G

U

A

G

U

U

G

G

G

A

C

C

U

U

G

U

C

C

C

U

G

U

G

A

G

A

G

U

A

G

G

A

C

G

U

C

G

C

C

A

A

G

C

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Flavobacterium

U

A

A

G

G

U

G

G

U

U

A

U

U

G

C

G

G

C

G

G

G

G

C

U

C

G

A

C

C

U

C

U

U

C

C

C

A

U

C

C

C

G

A

A

C

A

G

A

G

U

A

G

U

U

A

A

G

C

C

C

G

C

C

U

G

C

G

C

A

G

A

U

G

G

U

A

C

U

G

C

A

G

U

U

A

U

G

U

G

G

G

A

G

A

G

U

A

U

G

U

C

G

U

C

G

C

C

U

U

U

C

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Agrobacterium

C

G

A

C

C

U

G

G

U

G

G

U

C

A

U

C

G

C

G

G

G

G

C

G

G

C

U

G

C

A

C

C

C

G

U

U

C

C

C

U

U

U

C

C

G

A

A

C

A

C

G

G

C

C

G

U

G

A

A

A

C

G

C

C

C

C

A

G

C

G

C

C

A

A

U

G

G

U

A

C

U

U

C

G

U

C

U

C

A

A

G

A

C

G

C

G

G

G

A

G

A

G

U

A

G

G

U

C

G

C

U

G

C

C

A

G

G

U

C

U

-

-

-

-

-

-

-

-

-

-

-

Campylobacter

A

U

G

U

C

C

G

U

G

A

U

U

A

U

A

C

A

G

A

U

G

U

G

G

A

A

A

C

G

C

C

U

U

G

C

U

C

C

A

U

C

C

C

G

A

A

C

C

A

A

G

A

A

G

C

U

A

A

G

C

A

C

A

U

C

G

U

G

G

G

U

G

A

U

G

A

U

A

C

U

A

C

G

C

C

U

U

A

C

U

G

G

C

A

G

G

G

G

G

A

A

A

G

U

A

G

C

U

C

A

U

U

G

C

G

G

A

C

U

U

-

-

-

-

-

-

-

-

-

-

-

Escherichia

U

G

C

C

U

G

G

C

G

G

C

C

G

U

A

G

C

G

C

G

G

U

G

G

U

C

C

C

A

C

C

U

G

A

C

C

C

C

A

U

G

C

C

G

A

A

C

U

C

A

G

A

A

G

U

G

A

A

A

C

G

C

C

G

U

A

G

C

G

C

C

G

A

U

G

G

U

A

G

U

G

U

G

G

G

G

U

C

U

C

C

C

C

A

U

G

C

G

A

G

A

G

U

A

G

G

G

A

A

C

U

G

C

C

A

G

G

C

A

U

-

-

-

-

-

-

-

-

-

-

-

Thermotoga

A

U

C

C

C

C

G

G

G

U

G

C

C

G

A

U

A

C

U

G

G

G

G

C

G

G

G

A

A

A

C

A

C

C

C

G

G

U

U

C

C

A

U

U

C

C

G

A

A

C

C

C

G

G

C

C

G

U

U

A

A

G

C

C

G

C

C

C

U

G

G

G

C

C

G

A

U

G

G

U

A

G

U

A

U

G

G

G

G

G

C

A

G

C

C

C

C

A

U

G

C

G

A

G

A

G

U

A

G

G

U

A

G

C

G

C

C

C

G

G

G

G

A

U

-

-

-

-

-

-

-

Thermus

A

A

U

C

C

C

C

C

G

U

G

C

C

C

A

U

A

G

C

G

G

C

G

U

G

G

A

A

C

C

A

C

C

C

G

U

U

C

C

C

A

U

U

C

C

G

A

A

C

A

C

G

G

A

A

G

U

G

A

A

A

C

G

C

G

C

C

A

G

C

G

C

C

G

A

U

G

G

U

A

C

U

G

G

G

C

G

G

G

C

G

A

C

C

G

C

C

U

G

G

G

A

G

A

G

U

A

G

G

U

C

G

G

U

G

C

G

G

G

G

G

A

U

-

-

-

-

-

-

-

-

Methanothermobacter

G

U

U

G

G

C

G

G

U

C

A

U

G

G

C

G

U

G

G

G

G

U

U

U

A

U

A

C

C

U

G

A

U

C

U

C

G

U

U

U

C

G

A

U

C

U

C

A

G

U

A

G

U

U

A

A

G

U

C

C

U

G

C

U

G

C

G

U

U

G

U

G

G

G

U

G

U

G

U

A

C

U

G

C

G

G

U

U

U

U

U

U

G

C

U

G

U

G

G

G

A

A

G

C

C

C

A

C

U

U

C

A

C

U

G

C

C

A

G

A

C

A

A

-

-

-

-

-

-

-

-

Methanospirillum

U

C

A

A

U

A

G

C

G

G

C

C

A

C

A

G

C

A

G

G

U

G

U

G

U

C

A

C

A

C

C

C

G

U

U

C

C

C

A

U

U

C

C

G

A

A

C

A

C

G

G

A

A

G

U

U

A

A

G

A

C

A

C

C

U

C

A

C

G

U

G

G

A

U

G

A

C

G

G

U

A

C

U

G

A

G

G

U

A

C

G

C

G

A

G

U

C

C

U

C

G

G

G

A

A

A

U

C

A

U

C

C

U

C

G

C

U

G

C

U

A

U

U

G

U

U

-

-

-

-

-

Nanoarchaeum

G

U

U

U

C

G

U

G

G

G

A

G

G

G

C

C

A

U

A

G

C

G

G

C

C

C

G

G

G

A

A

C

C

A

C

C

C

G

U

A

C

C

C

A

U

C

U

C

G

A

A

C

A

C

G

G

A

A

G

U

U

A

A

G

C

C

G

G

G

C

C

G

C

G

U

C

C

C

G

A

G

U

G

G

U

A

C

U

G

C

C

C

C

G

C

G

A

A

G

G

G

G

U

G

G

G

A

A

G

C

U

C

G

G

G

A

U

G

C

C

C

-

-

-

-

-

-

-

-

-

-

Thermoplasma

G

G

C

A

A

C

G

G

U

C

A

U

A

G

C

A

G

C

A

G

G

G

A

A

A

C

A

C

C

A

G

A

U

C

C

C

A

U

U

C

C

G

A

A

C

U

C

G

A

C

G

G

U

U

A

A

G

C

C

U

G

C

U

G

C

G

U

A

U

U

G

C

G

U

U

G

U

A

C

U

G

U

A

U

G

C

C

G

C

G

A

G

G

G

U

A

C

G

G

G

A

A

G

C

G

C

A

A

U

A

U

G

C

U

G

U

U

A

C

C

A

C

U

-

-

-

-

-

-

-

-

Methanococcus

U

G

A

C

A

U

A

A

C

G

G

U

C

A

A

A

G

C

G

G

A

G

G

U

G

U

A

A

C

A

U

C

C

G

A

U

C

C

C

A

U

C

C

C

G

A

U

C

U

C

G

G

A

A

A

U

U

A

A

G

C

C

C

U

C

C

A

G

C

G

A

U

U

C

U

U

U

A

A

G

U

A

C

U

G

C

U

A

U

C

U

A

G

U

G

G

G

A

A

C

A

A

A

G

U

G

A

C

G

C

C

G

U

U

A

G

U

C

A

C

-

-

-

-

-

-

-

-

-

-

-

Thermococcus

G

G

U

A

C

G

G

C

G

G

U

C

A

U

A

G

C

G

G

C

G

G

G

G

U

C

A

C

A

C

C

C

G

G

U

C

U

C

G

U

U

U

C

G

A

C

C

C

C

G

G

A

A

G

U

U

A

A

G

C

C

C

G

C

C

A

G

C

G

A

U

C

C

C

G

G

U

U

G

U

A

C

U

G

C

C

C

U

C

C

G

G

G

A

G

G

G

G

G

C

G

G

G

A

A

C

C

G

G

G

G

A

C

G

C

C

G

C

C

G

G

C

C

A

C

-

-

-

-

-

-

Pyrobaculum

G

C

C

C

C

U

A

A

C

C

C

G

G

C

C

A

U

A

G

G

C

G

C

C

G

G

U

G

A

U

A

C

G

C

C

C

G

G

U

C

U

C

A

U

C

A

G

A

A

C

C

C

G

G

A

A

G

C

U

A

A

G

G

C

C

G

G

C

G

C

C

G

C

G

C

U

C

G

G

G

A

G

U

A

C

U

G

G

G

C

U

C

C

G

C

G

A

G

G

G

C

C

C

G

G

G

A

A

A

C

C

G

G

C

G

U

G

C

U

G

G

G

A

G

G

G

G

G

C

U

U

Sulfolobus

G

C

C

C

A

C

C

C

G

G

U

C

A

C

A

G

U

G

A

G

C

G

G

G

C

A

A

C

A

C

C

C

G

G

A

C

U

C

A

U

U

U

C

G

A

A

C

C

C

G

G

A

A

G

U

U

A

A

G

C

C

G

C

U

C

A

C

G

U

U

A

G

U

G

G

G

G

C

C

G

U

G

G

A

U

A

C

C

G

U

G

A

G

G

A

U

C

C

G

C

A

G

C

C

C

C

A

C

U

A

A

G

C

U

G

G

G

A

U

G

G

G

U

U

U

U

-

-

-

-

-

Halorubrum

G

U

A

G

G

C

G

G

C

C

A

A

A

G

C

G

G

C

A

G

G

G

A

A

A

G

A

C

C

C

G

U

A

C

C

C

A

U

U

C

C

G

A

A

C

A

C

G

G

A

A

G

U

G

A

A

G

C

C

U

G

C

C

A

G

C

G

U

A

C

C

A

G

C

U

A

G

U

A

C

U

G

G

A

G

U

G

G

G

A

G

A

C

C

C

U

C

U

G

G

G

A

G

C

G

C

U

G

G

U

U

C

G

C

C

G

C

C

U

A

C

U

C

-

-

-

-

-

-

-

-

Cryptosporidium

G

C

A

A

U

C

G

G

C

C

A

U

A

C

U

A

C

A

U

C

G

A

A

U

A

U

A

C

C

G

G

A

U

C

C

C

A

U

C

A

G

A

A

C

U

C

C

G

A

A

G

U

C

A

A

G

C

G

G

U

G

U

A

A

G

G

U

U

C

G

G

U

U

A

G

U

A

C

U

A

G

G

G

U

G

G

G

G

G

A

C

C

A

C

C

U

G

G

G

A

A

G

A

C

C

A

G

A

A

U

G

U

C

G

A

U

C

G

C

C

U

C

-

-

-

-

-

-

-

-

-

Candida

G

G

U

U

G

C

G

G

C

C

A

U

A

U

C

U

A

G

C

A

G

A

A

A

G

C

A

C

C

G

U

U

C

C

C

C

G

U

U

C

G

A

U

C

A

A

C

C

G

U

A

G

U

U

A

A

G

C

U

G

C

U

A

A

G

A

G

C

A

A

U

A

C

C

G

A

G

U

A

G

U

G

U

A

G

U

G

G

G

A

G

A

C

C

A

U

A

C

G

C

G

A

A

A

C

U

A

U

U

G

U

G

C

U

G

C

A

A

U

C

U

-

-

-

-

-

-

-

-

-

-

Drosophila

G

C

C

A

A

C

G

A

C

C

A

U

A

C

C

A

C

G

C

U

G

A

A

U

A

C

A

U

C

G

G

U

U

C

U

C

G

U

C

C

G

A

U

C

A

C

C

G

A

A

A

U

U

A

A

G

C

A

G

C

G

U

C

G

G

G

C

G

C

G

G

U

U

A

G

U

A

C

U

U

A

G

A

U

G

G

G

G

G

A

C

C

G

C

U

U

G

G

G

A

A

C

A

C

C

G

C

G

U

G

U

U

G

U

U

G

G

C

C

U

-

-

-

-

-

-

-

-

-

-

-

Euglena

G

A

G

U

A

C

G

G

C

C

A

U

A

C

U

A

C

C

G

G

G

A

A

U

A

C

A

C

C

U

G

A

A

C

C

C

G

U

U

C

G

A

U

U

U

C

A

G

A

A

G

U

U

A

A

G

C

C

G

G

G

U

U

A

G

G

C

C

C

A

G

U

U

A

G

U

A

C

U

G

A

G

U

G

G

G

C

G

A

C

C

A

C

U

U

G

G

G

A

A

C

A

C

U

G

G

G

U

G

C

U

G

U

A

C

G

C

U

U

-

-

-

-

-

-

-

-

-

-

-

-

Homo

G

U

C

U

A

C

G

G

C

C

A

U

A

C

C

A

C

C

C

U

G

A

A

C

G

C

G

C

C

C

G

A

U

C

U

C

G

U

C

U

G

A

U

C

U

C

G

G

A

A

G

C

U

A

A

G

C

A

G

G

G

U

C

G

G

G

C

C

U

G

G

U

U

A

G

U

A

C

U

U

G

G

A

U

G

G

G

A

G

A

C

C

G

C

C

U

G

G

G

A

A

U

A

C

C

G

G

G

U

G

C

U

G

U

A

G

G

C

U

U

U

-

-

-

-

-

-

-

-

-

-

Nosema

G

A

U

A

C

G

G

U

U

A

U

A

U

U

U

A

C

U

G

A

A

A

A

C

C

A

C

C

G

G

A

A

C

C

C

A

U

C

A

G

A

A

C

U

C

C

G

A

A

G

U

U

A

A

A

C

C

A

G

U

A

U

G

A

G

C

U

U

A

A

U

U

A

G

U

A

C

U

A

A

G

A

A

G

G

G

A

G

A

C

C

A

C

U

U

G

G

G

A

A

C

G

U

U

G

G

G

U

G

G

U

A

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

Zea

G

G

A

U

G

C

G

A

U

C

A

U

A

C

C

A

G

C

A

C

U

A

A

A

G

C

A

C

C

G

G

A

U

C

C

C

A

U

C

A

G

A

A

C

U

C

C

G

A

A

G

U

U

A

A

G

C

G

U

G

C

U

U

G

G

G

C

G

A

G

A

G

U

A

G

U

A

C

U

A

G

G

A

U

G

G

G

U

G

A

C

C

U

C

C

U

G

G

G

A

A

G

U

C

C

U

C

G

U

G

U

U

G

C

A

U

U

C

C

-

-

-

-

-

-

-

-

-

-

-

-

Caenorhabditis

G

C

U

U

A

C

G

A

C

C

A

U

A

U

C

A

C

G

U

U

G

A

A

U

G

C

A

C

G

C

C

A

U

C

C

C

G

U

C

C

G

A

U

C

U

G

G

C

A

A

G

U

U

A

A

G

C

A

A

C

G

U

U

G

A

G

U

C

C

A

G

U

U

A

G

U

A

C

U

U

G

G

A

U

C

G

G

A

G

A

C

G

G

C

C

U

G

G

G

A

A

U

C

C

U

G

G

A

U

G

U

U

G

U

A

A

G

C

U

-

-

-

-

-

-

-

-

-

-

-

-

Dictyostelium

G

U

A

U

A

C

G

G

C

C

A

U

A

C

U

A

G

G

U

U

G

G

A

A

A

C

A

C

A

U

C

A

U

C

C

C

G

U

U

C

G

A

U

C

U

G

A

U

A

A

G

U

A

A

A

U

C

G

A

C

C

U

C

A

G

G

C

C

U

U

C

C

A

A

G

U

A

C

U

C

U

G

G

U

U

G

G

A

G

A

C

A

A

C

A

G

G

G

G

A

A

C

A

U

A

G

G

G

U

G

C

U

G

U

A

U

A

C

U

-

-

-

-

-

-

-

-

-

-

-

-