1........10........20........30........40........50........60........70........80........90.......100.......110.......120.......130.......140.......150.......160.......170.......180.......190.......200.......210.......220.......230.......240.......250.......260.......270.......280.......290.......300.......310.......320.......330.......340.......350.....
hemo-tunicate-1 ----M--------------------------------P--------------------------------------------------------------FTDEELKLLRDSWDEVK--KLGM---KEVGLHIFTGLLNAAPSLRTLFYTIDLPD--E--EE---L-TIDVMR-EN-----KKVVAHATRIANAISKFIKFLD---Q-PD---E-LEKLLT---SLGESH-ARRQ---V-DPESFEYVAPVILSVIGGH-L----K----LPSNSPTLQAWVKA----YGVLRNGIV-S--AMEA---------------------------------------------------
hemo-tunicate-3 ----M--------------------------------S--------------------------------------------------------------LTSEQVVLLRSSWQTIG--KLGM---SNVGLAVLHRLFNDVPETLPFFHSVLSP---T--QQ---T-EIEVLK-SN-----AKVVRHASRVGLSIDKIINLLD---N-GE---E-LVKYLL---FLGQVH-VKRS---I-PRKYFSAMGPVLLSVISAV-L----E----KDLDAPVMQAWATA----YGVIEQGII-D--GM-----------------------------------------------------
hemo-polychaete-4 ----M--------------------------------G--------------------------------------------------------------LSAAQRQVVASTWKDIAGSD-NG---AGVGKECFTKFLSAHHDIAAVFG--------F--SG---A-S-------D-----PGVADLGAKVLAQIGVAVSHLG---D-EG---K-MVAEMK---AVGVRH-KGYGYKHI-KAEYFEPLGASLLSAMEHR-I----G----GKMTAAAKDAWAAA----YADISGALI-S--GL--QS-------------------------------------------------
myo-polychaete ----MA-------------------------------G--------------------------------------------------------------LSGADIAVIRSTWAKVQGSG-SA---TDIGRSIFIKFFELDPAAQNEFP--------C--KG---E-SLAALK-TN-----VLLGQHGAKFMEYITTAVNGLD---DYAG---K-AHGPLT---ELGSRH-KTRG---T-TPANFGKAGEALLAILASV-V----G----GDFTPAAKDAWTKV----YNTISSTMQ-A--AL-----------------------------------------------------
leghemo-pea ----M--------------------------------G--------------------------------------------------------------FTEKQEALVNSSWELFK--Q-NP---SY-SVLFYTIILKKAPAAKGMFS--------F--LK---D-SA-EVV-DS-----PKLQAHAEKVFGMVHDSAIQLR---A-SG---E-VVLGDAT---LGAIH-IQKG---V-VDPHFVVVKEALLETIKEA-S----G----EKWSEELSTAWEVA----YEGLASAIK-K--AM----N------------------------------------------------
leghemo-rice ----M--------------------------------ALVEDNNAVAVS---------------------------------------------------FSEEQEALVLKSWAILK--K-DS---ANIALRFFLKIFEVAPSASQMFS--------F--LR---N-SDVPLE-KN-----PKLKTHAMSVFVMTCEAAAQLR---K-AG---K-VTVRDTTLKRLGATH-LKYG---V-GDAHFEVVKFALLDTIKEEVP----A----DMWSPAMKSAWSEA----YDHLVAAIK-Q--EM----KPAE---------------------------------------------
neuro-human ----M-E------------------------------R-----------------------------------------------------------------PEPELIRQSWRAVS--R-SP---LEHGTVLFARLFALEPDLLPLFQ--------Y--NCRQFS-SPEDCL-SS-----PEFLDHIRKVMLVIDAAVTNVE---D-LS---S-LEEYLA---SLGRKH-RAVG---V-KLSSFSTVGESLLYMLEKC-L----G----PAFTPATRAAWSQL----YGAVVQAMS-R--GW----------DGE----------------------------------------
neuro-zebrafish ----M-E------------------------------K--------------------------------------------------------------LSEKDKGLIRDSWESLG--K-NK---VPHGIVLFTRLFELDPALLTLFS--------YSTNC---G-DAPECL-SS-----PEFLEHVTKVMLVIDAAVSHLD---D-LH---T-LEDFLL---NLGRKH-QAVG---V-NTQSFALVGESLLYMLQSS-L----G----PAYTTSLRQAWLTM----YSIVVSAMT-R--GW--------AKNGEHKSN------------------------------------
HbA-blood_clam ----M--------------------------------A-----------DEEQIAKAVFKEAKALC----------------------------------DSDAAKENLRSSFKILA--N-DI---ESSGLQLMKNLFTSHPDTLGYFA------R-F--KD---V-SLG--K-QN-----WQLRGHAITLMYALMNFVDALD---E-PD---R-LKCVVL---KFAVNH-NNRS---I-SPQVFGKINGPMDLLLKQR-M----G----KYYNRETANAWKQL----VGVVQAALT-T--PN-----------------------------------------------------
HbI-Lucina ----M--------------------------------S--------------------------------------------------------------LSAAQKDNVKSSWAKAS--A-AW---GTAGPEFFMALFDAHDDVFAKFS------GLF--KG---A-AKGTVK-NT-----PEMAAQAQSFKGLVSNWVDNLD---N-AG---A-LEGQCK---TFAANH-KARG---I-SAGQLEAAFKVLAGFMKSY------G---------GDEGAWTAV----AGALMGMIR-P--NM-----------------------------------------------------
HbII-Lucina ----T--------------------------------T--------------------------------------------------------------LTNPQKAAIRSSWSKFM--D-NG---VSNGQGFYMDLFKAHPETLTPFK------SLF--GG---L-TLAQLQ-DN-----PKMKAQSLVFCNGMSSFVDHLD---D--N---M-LVVLIQ---KMAKLH-NNRG---I-RASDLRTAYDILIHYMEDH--NHMVG---------GAKDAWEVF----VGFICKTLG-D--YM-----------------KELS--------------------------------
myo-Dolabella -------------------------------------A--------------------------------------------------------------LSAAEAEVVAKSWGPVF--A-NK---DANGDNFLIALFEAYPDSPNFFA------D-F--KG---K-SIADIR-AS-----PKLRNVSSRIVSRLNEFVSSAA---D-AG---K-MAAMLD---QFSKEH-AGFG---V-GSQQFQNVSAMFPGFVASI-A-----AP-----PAGADAAWGKL----FGLIIDAMKKA--GK-----------------------------------------------------
myo-snail -------------------------------------S--------------------------------------------------------------LQPASKSALASSWKTLA--K-DAATIQNNGATLFSLLFKQFPDTRNYFT------H-F--GN---M-SDAEMK-TT-----GVGKAHSMAVFAGIGSMIDSMD---D-AD---C-MNGLAL---KLSRNH-IQRK---I-GASRFGEMRQVFPNFLDEA-L----G----GGASGDVKGAWDAL----LAYLQDNKQAQ--AL-----------------------------------------------------
drosophila ----M------------------------------------------------------------------------------------------------NSDEVQLIKKTWEIPV--A-TP---TDSGAAILTQFFNRFPSNLEKFP--------F--RD---V-PLEELS-GN-----ARFRAHAGRIIRVFDESIQVLGQDGD-LE---K-LDEIWT---KIAVSH-IPRT---V-SKESYNQLKGVILDVLTAA-C----S----LDES--QAATWAKLVDHVYAIIFKAID-DDGNA-----------------------------------------------------
midge-8 AVTPM------------------------------------------------------------------------------------------------SADQLALFKSSWNTVK--H-NE-------VDILYAVFKANPDIQAKFP------Q-F--AG---K-DLDSIK-DS-----ADFAVHSGRIVGFFSEVIGLIG---N-PENRPA-LKTLID---GLASSH-KARG---I-EKAQFEEFRASLVDYLSHH-L----D----WNDT--MKSTWDLALNNMFFYILHALE-V--AQ-----------------------------------------------------
honeybee ----M--GTFLRFLGISSSDDNRIDQAT---------G--------------------------------------------------------------LTERQKKLVQNTWAVVR--K-DE---VASGIAVMTAFFKKYPEYQRYFT------A-F--MD---T-PLNELP-AN-----KRFQAHCAGVITALNNVIDFLH---D-PG---L-MEASLI---GLVERH-KKRG---Q-TKEEFQNLKEVMLEVLRQA-L----G----KQYTPEVAEAWNKTLDMMFGKIYQVFA-S---------------------------------------------------------
beetle ----M--GQLISLYKYYTTRTDDPDPLT---------T--------------------------------------------------------------LTSREVFLVQSSWDPIK--K-DL---TGYGVQLLLFLFKKYPEEQQNFP--------F--RD---M-PFEELG-AS-----KKFHAHCSNVMYAVDSIIDSLK---D-GE---L-LVNILE---KIGRNH-HRNT---V-KPISFWHVKETMLEFFKKM-M------------NDETLKAWDKALQVAFGVVAKELD-K--KN-----------------------------------------------------
cyto-beetle ----M--GIITSTLSYYMTKTNDPDPIT---------G--------------------------------------------------------------LTSRDRYVIQTSWAPVK--K-DL---TGNGVALLLLYFEKFPATKNYFV--------F--KD---V-PNEKLK-TD-----KKFHAHCNSVMVTLDSLIANLN---D-GE---L-IVSLLE---KLGKNH-KRHG---I-KDDAYDQLKETVIELFSSF-M------------TKEELETWDKLLKVAFSVIIKYL------------------------------------------------------------
cyto-fish ----M--------------------------------S----------------------------HREPPPPQLAVQRRDVDGQDGPERAEP-------LSDTEREMIRDAWGHVY--K-NC---EDVGVSILIRFFVNFPSAKQYFS------Q-F--QD---MEEPEEME-RS-----SQLRHHACRVMNALNTVVENLH---D-PE---K-VSSVLA---VVGRAHAVKHK---V-EPMYFKILSGVILEVLCED-F----P----EFFTADVQLVWSKL----MATVYWHVT-G--AY---------------------TDVGWLQ-----------VSSSAV--------
cyto-human ----M---------------------------------------------------------------EKVPGEMEIERR--------ERSEE-------LSEAERKAVQAMWARLY--A-NC---EDVGVAILVRFFVNFPSAKQYFS------Q-F--KH---MEDPLEME-RS-----PQLRKHACRVMGALNTVVENLH---D-PD---K-VSSVLA---LVGKAHALKHK---V-EPVYFKILSGVILEVVAEE-F----A----SDFPPETQRAWAKL----RGLIYSHVT-A--AY---------------------KEVGWVQQVPNATTPPATLPSSGP--------
hagfish ----M--------------------------------S-------------------------------------------------------AHGIAR-TTEGERAAVRASWAVLM--K-DY---EHAGVQILDKFFKANPAAKPFFT------K-M--KD---LHTLEDLA-SS-----ADARWHVERIIQAVNFAVINIE---D-RE---K-LSNKFV---KLSQDHIEEFH---VTDPQYFMILSQTILDEV----E----K--RNGGLSGEGKSGWHKV----MTIICKMLK-S--KY-----------------------------------------------------
lamprey ----M-----------------------PIVDSGSVPA--------------------------------------------------------------LTAAEKATIRTAWAPVY--A-KY---QSTGVDILIKFFTSNPAAQAFFP------K-F--QG---LTSADQLK-KS-----MDVRWHAERIINAVNDAVVAMD---D-TE---K-MSLKLR---ELSGKHAKSFQ---V-DPQYFKVLAAVIVDTV----L----P----GD------AGLEKL----MSMICILLR-S--SY-----------------------------------------------------
myo-human ----M--------------------------------G--------------------------------------------------------------LSDGEWQLVLNVWGKVE--A-DI---PGHGQEVLIRLFKGHPETLEKFD------K-F--KH---LKSEDEMK-AS-----EDLKKHGATVLTALGGILKKKG-----------HHEAEIK---PLAQSHATKHK---I-PVKYLEFISECIIQVLQSK-H----P----GDFGADAQGAMNKA----LELFRKDMA-S--NY---------------------------------------------KELGFQG-
myo-shark -------------------------------------------------------------------------------------------------------VDWEKVNSVWSAVE--S-DL---TAIGQNILLRLFEQYPESQNHFP------K-F--KN----KSLGELK-DT-----ADIKAQADTVLSALGNIVKKKG-----------SHSQPVK---ALAATHITTHK---I-PPHYFTKITTIAVDVLSEM-Y----P----SEMNAQVQAAFSGA----FKIICSDIE-K--EY---------------------------------------------KAANFQG-
hemo-alpha-human ----M--------------------------------V--------------------------------------------------------------LSPADKTNVKAAWGKVG--A-HA---GEYGAEALERMFLSFPTTKTYFP------H-F---D---LS------HGS-----AQVKGHGKKVADALTNAVAHVD---D--------MPNALS---ALSDLHAHKLR---V-DPVNFKLLSHCLLVTLAAH-L----P----AEFTPAVHASLDKF----LASVSTVLT-S--KY----------------------------------------------------R
hemo-alpha-shark -------------------------------------V--------------------------------------------------------------LSAADKTAIKHLTGSLR--T-NA---EAWGAESLARMFATTPSTKTYFS------K-F--TD---FS------ANG-----KRVKAHGGKVLNAVADATDHLD---N--------VAGHLD---PLAVLHGTTLC---V-DPHNFPLLTQCILVTLAAH-L---------TELKPETHCALDKF----LCEVATALG-S--HY----------------------------------------------------R
hemo-beta-human ----M--------------------------------V-------------------------------------------------------------HLTPEEKSAVTALWGKV-----NV---DEVGGEALGRLLVVYPWTQRFFE------S-F--GD---LSTPDAVM-GN-----PKVKAHGKKVLGAFSDGLAHLD---N--------LKGTFA---TLSELHCDKLH---V-DPENFRLLGNVLVCVLAHH-F----G----KEFTPPVQAAYQKV----VAGVANALA-H--KY----------------------------------------------------H
hemo-beta-shark -------------------------------------V-------------------------------------------------------------HWTGEEKALVNAVWTKT-----DH---QAVVAKALERLFVVYPWTKTYFV------K-F--NG---K----------FHASDSTVQTHAGKVVSALTVAYNHID---D--------VKPHFV---ELSKKHYEELH---V-DPENFKLLANCLEVELGHA-L----H----KEFTPEVQAAWSKF----SNVVVDALS-K--GY----------------------------------------------------H